Effect of Catfish (Clarias batrachus) Flour and Pre-Gelatinized Red Bean (Vigna angularis) Flour Substitution on the Saturated and Trans Fat Content of Dried Sponge Cake

Authors

  • Dian Maulida Ahsan Universitas Muhammadiyah Surakarta
  • Fitriana Mustikaningrum Universitas Muhammadiyah Surakarta

DOI:

https://doi.org/10.71435/738211

Keywords:

Hypertension, Risk Catfish Flour, Pregelatinized Red Bean Flour, Dried Sponge Cake

Abstract

Stunting prevention requires not only nutritional intervention but also the development of practical, acceptable, and locally based functional food products for pregnant women. This study aimed to analyze the effect of substituting catfish flour (Clarias batrachus) and pregelatinized red bean flour (Vigna angularis) in dried sponge cake on saturated fat and trans-fat content, while positioning the product as a potential value-added innovation in maternal nutrition management. This study used an experimental design with a Completely Randomized Design consisting of three formulations and two replications. Formula A served as the control with 100% wheat flour, Formula B used 15% catfish flour and 20% pregelatinized red bean flour, and Formula C used 25% catfish flour and 10% pregelatinized red bean flour. Saturated fat and trans-fat contents were analyzed using laboratory fat analysis procedures, and the data were processed using the Kruskal-Walli’s test. The results showed that saturated fat content decreased descriptively in the substitution formulas, with the lowest value found in Formula B at 2.91%, although the difference was not statistically significant (p>0.05). Trans fat content showed a significant difference among formulations (p<0.05), with Formula B producing the lowest value at 16.81%. These findings indicate that the combination of catfish flour and pregelatinized red bean flour, especially Formula B, can improve the lipid quality of dried sponge cake. The product has potential as a healthier local functional food innovation that supports maternal nutrition, food diversification, and stunting prevention strategies. Further sensory, shelf-life, and feasibility studies are needed before commercialization.

References

Ali, M. R., Mohameden, H. A., & Mohamed, R. M. (2026). Valorization of Waste and Microbial Resources for Sustainable Edible Oil Production: Nutritional Enhancement and Circular Bioeconomy Approaches. Sustainability, 18(10), 4655. https://doi.org/10.3390/su18104655

Aminah, M., Mulyo, G. P. E., Amelia, S., Khoerunnisa, A., & Aeni, M. N. (2022). Potential Local Food Material Based On Flour (Catfish, Red Beans, And Kepok Banana) As An Alternative Snack For Young Women With Anemia. In International Conference On Interprofessional Health Collaboration And Community Empowerment (Vol. 4, No. 1, pp. 154-159). https://doi.org/10.34011/icihcce.v4i1.235

Arvianto, A. A., Swastawati, F., & Wijayanti, I. (2016). Pengaruh fortifikasi tepung daging ikan lele dumbo (Clarias gariepinus) terhadap kandungan asam amino lisin pada biskuit. Jurnal Pengolahan dan Bioteknologi Hasil Perikanan, 5(4), 20–25.

Astuti, R. W., Sitasari, A., Widyawati, H. E., & Rooiqoh, Q. F. (2023). Pengaruh tepung kacang merah (Phaseolus vulgaris L.) dan tepung sukun (Artocarpus communis) terhadap karakteristik organoleptik, kadar proksimat, serat pangan, dan kadar pati resisten pada snack bar sebagai pangan fungsional pada diabetisi. Medika Respati: Jurnal Ilmiah Kesehatan, 18(4), 269–278. https://doi.org/10.35842/mr.v18i4.910

Azwar, A., Saputra, N., & Mulia, R. A. (2026). Policy implementation challenges in stunting reduction: a bibliometric analysis of global research trends. Cogent Social Sciences, 12(1), 2624137. https://doi.org/10.1080/23311886.2026.2624137

Chanyarungrojn, P. A., Lelijveld, N., Crampin, A., Nkhwazi, L., Geis, S., Nyirenda, M., & Kerac, M. (2023). Tools for assessing child and adolescent stunting: Lookup tables, growth charts and a novel appropriate-technology “MEIRU” wallchart‐a diagnostic accuracy study. PLOS Global Public Health, 3(7), e0001592. https://doi.org/10.1371/journal.pgph.0001592

Faridah, D. N., Silitonga, R. F., Indrasti, D., Afandi, F. A., Jayanegara, A., & Anugerah, M. P. (2022). Verification of autoclaving-cooling treatment to increase the resistant starch contents in food starches based on meta-analysis result. Frontiers in Nutrition, 9, 904700. https://doi.org/10.3389/fnut.2022.904700

Farooq, M. A., & Yu, J. (2024). Recent advances in physical processing techniques to enhance the resistant starch content in foods: A review. Foods, 13(17), 2770. https://doi.org/10.3390/foods13172770

Gantner, M., & Kostyra, E. (2023). Special issue on the latest research on flavor components and sensory properties of food during processing and storage. Foods, 12(20), 3761. https://doi.org/10.3390/foods12203761

Gebru, S. B., Hailu, T. S., & Taffere, G. R. (2023). Food safety knowledge, attitude, and practice of food handlers at food service establishments in southern Tigray, Ethiopia. Global Social Welfare, 10(3), 249-262. https://doi.org/10.1007/s40609-023-00284-9

Ghosh, S., Sarkar, T., & Chakraborty, R. (2024). Functional food and food innovation toward quality and safety regulations. Food Coatings and Preservation Technologies, 585-609. https://doi.org/10.1002/9781394237623.ch17

Hart, S. L. (1995). A natural-resource-based view of the firm. Academy of management review, 20(4), 986-1014. https://doi.org/10.2307/258963

Hasanah, F. (2020). Substitusi tepung ikan lele (Clarias sp.) pada pembuatan cookies sebagai makanan ringan. Prosiding Pendidikan Teknik Boga Busana, 15(1). https://jurnal.uny.ac.id/index.php/ptbb/article/view/35945

Kabahenda, M. K., & Stoecker, B. J. (2024). Associations between maternal dietary intake and nutritional status with fetal growth at 14 to 26 weeks gestation: a cross-sectional study. BMC nutrition, 10(1), 77. https://doi.org/10.1186/s40795-024-00885-3

Karlsson, O., Kim, R., & Bogin, B. (2022). Maternal height-standardized prevalence of stunting in 67 low-and middle-income countries. Journal of Epidemiology, 32(7), 337-344. https://doi.org/10.2188/jea.je20200537

Ketkaew, C., Oktaviani, R. D., Padthar, S., Leeanansaksiri, R., Meethonglang, T., & Hanna, A. (2024). Enhancing sustainable food innovation through consumer participatory co-creation: A case study on plant-based dietary snack bars in Thailand. Future Foods, 10, 100433. https://doi.org/10.1016/j.fufo.2024.100433

Khaire, A., Wadhwani, N., Madiwale, S., & Joshi, S. (2020). Maternal fats and pregnancy complications: Implications for long-term health. Prostaglandins, Leukotrienes and Essential Fatty Acids, 157, 102098. https://doi.org/10.1016/j.plefa.2020.102098

Kocatepe, D., Turan, H., Taşkaya, G., Kaya, Y., Erden, R., & Erdoğdu, F. (2011). Effects of cooking methods on the proximate composition of Black Sea anchovy (Engraulis encrasicolus, Linnaeus 1758). Gıda, 36(2), 71–75.

Lehmann, U., Jacobasch, G., & Schmiedl, D. (2002). Resistant starch in different dietary samples. Journal of Agricultural and Food Chemistry, 50(18), 5236–5240. https://doi.org/10.1021/jf020193f

Mardalena, I. (2017). Dasar-dasar ilmu gizi dalam keperawatan: Konsep dan penerapan pada asuhan keperawatan. http://eprints.poltekkesjogja.ac.id/7975/1/BUKU%20DASAR-DASAR%20ILMU%20GIZI%20DALAM%20KEPERAWATAN.pdf

Marine, B. T., Haile, Y. A., & Zewde, M. G. (2026). Maternal nutrition practices and its implications for child growth and development. Scientific Reports. https://doi.org/10.1038/s41598-026-37151-4

Mathew, J., Blossom, L., Gopal, T. S., & Thomas, A. (2022). Nutritional and quality properties of pasta and noodles incorporated with fish/and fishery-derived ingredients using extrusion technology-A review. Journal of Aquatic Food Product Technology, 31(9), 1002-1023. https://doi.org/10.1080/10498850.2022.2120376

Mititelu, M., Lupuliasa, D., Neacșu, S. M., Olteanu, G., Busnatu, Ș. S., Mihai, A., ... & Scafa-Udriște, A. (2024). Polyunsaturated fatty acids and human health: a key to modern nutritional balance in association with polyphenolic compounds from food sources. Foods, 14(1), 46. https://doi.org/10.3390/foods14010046

Moelyo, A. G., Sitaresmi, M. N., & Julia, M. (2025). Growth faltering or deceleration toward target height: Linear growth interpretation using WHO growth standard 2006 for Indonesian children. Plos one, 20(4), e0290053. https://doi.org/10.1371/journal.pone.0290053

Mustikaningrum, F. (2011). Pengaruh pratanak kacang kapri (Pisum sativum axiphium L.) terhadap kadar serat pangan dan pati resisten serta sifat hipoglikemiknya pada tikus diabetik induksi alloksan (Skripsi, Universitas Gadjah Mada). https://etd.repository.ugm.ac.id/penelitian/detail/51706

Nayak, B., Bhattacharyya, S. S., & Krishnamoorthy, B. (2023). Integrating the dialectic perspectives of resource-based view and industrial organization theory for competitive advantage–a review and research agenda. Journal of Business & Industrial Marketing, 38(3), 656-679. https://doi.org/10.1108/JBIM-06-2021-0306

Nwankwo, M., Danborno, B., Musa, S. A., & Akuyam, A. S. (2024). Prevalence and associated factors of stunting and thinness among children and adolescents in Nasarawa State: comparison with World Health Organization reference. Rwanda Medical Journal, 81(1), 23-37. https://doi.org/10.4314/rmj.v81i1.3

Prameswary, A. I., Pranata, F. S., & Purwijantiningsih, L. M. E. (2022). Kualitas bolu klemben dengan substitusi tepung tempe kacang tunggak (Vigna unguiculata) dan tepung umbi talas (Xanthosoma sagittifolium). Jurnal Teknologi Pangan dan Gizi (Journal of Food Technology and Nutrition), 21(1), 1–11. https://doi.org/10.33508/jtpg.v21i1.3500

Rahman, T., Andriansyah, R. C. E., Luthfiyanti, R., Surahman, D. N., & Triyono, A. (2025, April). Analysis of nutrient and resistant starch content of modified suweg flour (Amorphophallus campanulatus B) by autoclaving-cooling cycles method. In IOP Conference Series: Earth and Environmental Science (Vol. 1477, No. 1, p. 012062). IOP Publishing.

Rasul, M. G., Yuan, C., Yu, K., Takaki, K., & Shah, A. K. M. A. (2022). Factors influencing the nutritional composition, quality and safety of dried fishery products. Food Research, 6(5), 444-466. https://doi.org/10.26656/fr.2017.6(5).730

Rezaeizadeh, G., Mansournia, M. A., Keshtkar, A., Farahani, Z., Zarepour, F., Sharafkhah, M., ... & Poustchi, H. (2024). Maternal education and its influence on child growth and nutritional status during the first two years of life: a systematic review and meta-analysis. EClinicalMedicine, 71. https://doi.org/10.1016/j.eclinm.2024.102574

Saadat, A., Siddique, M., & Hashim, M. M. (2024). Knowledge, attitude and practice (kap) study of food safety and hygiene practices of household consumers. Health Sciences Journal, 2(2), 80-86. https://doi.org/10.3390/foods11142090

Sartika, R. A. (2008). Pengaruh asam lemak jenuh, tidak jenuh, dan asam lemak trans terhadap kesehatan. Kesmas: Jurnal Kesehatan Masyarakat Nasional, 2(4), 154–160. https://doi.org/10.21109/kesmas.v2i4.258

Scheffler, C., & Hermanussen, M. (2022). Stunting is the natural condition of human height. American Journal of Human Biology, 34(5), e23693. https://doi.org/10.1002/ajhb.23693

Sharif, M. K., Sarwar, K., Abid, N., & Bashir, M. A. (2024). Food Security, Food Safety, and Sanitation. Food Security in the Developing World, 191-225.

Shenoy, S., Sharma, P., Rao, A., Aparna, N., Adenikinju, D., Iloegbu, C., ... & Peprah, E. (2023). Evidence-based interventions to reduce maternal malnutrition in low and middle-income countries: a systematic review. Frontiers in Health Services, 3, 1155928. https://doi.org/10.3389/frhs.2023.1155928

Syarbini, M. H., & Perkumpulan Ekskul Kuliner Indonesia. (n.d.). Referensi komplet A–Z bakery: Fungsi bahan, proses pembuatan roti, dan panduan menjadi bakepreneur. Kubuku. Retrieved April 5, 2026, from https://bacabuku.com/detail/referensi-komplet-a-z-bakery--fungsi-bahan--proses-pembuatan-roti--dan-panduan-menjadi-bakepreneur/84394

Varadarajan, R. (2023). Resource advantage theory, resource based theory, and theory of multimarket competition: does multimarket rivalry restrain firms from leveraging resource advantages?. Journal of Business Research, 160, 113713. https://doi.org/10.1016/j.jbusres.2023.113713

Vékony, B., Juhász, R., Hajas, L., Benedek, C., & Bodor, Z. (2026). Plant-Based Essential Fatty Acids and Ketogenic Vegan Diet. In Vegan Gastronomy and Sustainable Health (pp. 331-364). Apple Academic Press. https://doi.org/10.1201/9781998511891-18

Waluyo, W., Pranoto, Y., Sardjono, S., & Marsono, Y. (2021). Peningkatan pati resisten dan karakteristik tepung kacang merah (Phaseolus vulgaris L.) pratanak metode kombinasi pengukusan, oven microwave, autoklaf, dan pendinginan. Jurnal Nutrisia, 23(1), 32–43. https://doi.org/10.29238/jnutri.v23i1.217

Wang, Y., Han, J., Wang, D., Gao, F., Zhang, K., Tian, J., & Jin, Y. (2022). Research update on the impact of lactic acid bacteria on the substance metabolism, flavor, and quality characteristics of fermented meat products. Foods, 11(14), 2090.

Wira, W., Harini, N., Damat, D., Ariadi, B. Y., Savitri, E. S., Huu, N. N., ... & Sobach, I. N. (2025). Autoclaving cooling method for optimizing resistant starch content and physicochemical properties of modified tannia flour. Sarhad Journal of Agriculture, 39, 123-130. https://doi.org/10.17582/journal.sja/2023/39/s1.123.130

Xu, Y., Zhang, D., Schroyen, M., Li, X., Yang, Q., & Hou, C. (2026). Modulation of postmortem glycolysis in pork by electrostatic field: Roles of pyruvate kinase and myofibrillar protein phosphorylation. Food Chemistry, 148928. https://doi.org/10.1016/j.foodchem.2026.148928

Yisahak, S. F., Mumford, S. L., Grewal, J., Li, M., Zhang, C., Grantz, K. L., & Hinkle, S. N. (2021). Maternal diet patterns during early pregnancy in relation to neonatal outcomes. The American Journal of Clinical Nutrition, 114(1), 358–367. https://doi.org/10.1093/ajcn/nqab019

Zhao, L., Ju, W. M., Wang, L. L., Ye, Y. B., Liu, Z. Y., Cavender, G., ... & Sun, S. Q. (2025). Functional Ingredients: From Molecule to Market—AI-Enabled Design, Bioavailability, Consumer Impact, and Clinical Evidence. Foods, 14(17), 3141. https://doi.org/10.3390/foods14173141

Zupo, R., Castellana, F., Boero, G., Matera, E., Colacicco, G., Piscitelli, P., ... & Sardone, R. (2024). Processed foods and diet quality in pregnancy may affect child neurodevelopment disorders: a narrative review. Nutritional neuroscience, 27(4), 361-381. https://doi.org/10.1080/1028415x.2023.2197709

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Published

2026-06-29

How to Cite

Dian Maulida Ahsan, & Fitriana Mustikaningrum. (2026). Effect of Catfish (Clarias batrachus) Flour and Pre-Gelatinized Red Bean (Vigna angularis) Flour Substitution on the Saturated and Trans Fat Content of Dried Sponge Cake. Journal of Asian-African Focus in Health, 4(1), 143–160. https://doi.org/10.71435/738211